Human PROS (Protein S) ELISA Kit | ELK2722

Human PROS (Protein S) ELISA Kit | ELK2722 | Gentaur UK, US & Europe Distribution

Alternative Names: PSA; PS; PS26; PS21; PS22; PS23; PS24; PS25; VKDPS; VKDP-S; Protein Sa; Vitamin K Dependent Protein S

Species: Human

Assay Type: Sandwich

Sensitivity: 27.7 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: Plasma

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein S (PROS) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein S (PROS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein S (PROS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein S (PROS) in the samples is then determined by comparing the OD of the samples to the standard curve.