Human Protein amnionless (AMN) ELISA Kit | KTE62442

Human Protein amnionless (AMN) ELISA Kit | KTE62442 | Gentaur UK, US & Europe Distribution

Application: This Human Protein amnionless (AMN) ELISA Kit employs a two-site sandwich ELISA to quantitate AMN in samples. An antibody specific for AMN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAMN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Protein amnionless microplate
• Human Protein amnionless standard
• Human Protein amnionless detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Protein amnionless (AMN) ELISA Kit has high sensitivity and excellent specificity for detection of Human AMN. No significant cross-reactivity or interference between Human AMN and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Protein amnionless is is a type I transmembrane protein. It is thought to modulate bone morphogenetic protein (BMP) receptor function by serving as an accessory or coreceptor, and thus facilitates or hinders BMP binding. It is known that the mouse AMN gene is expressed in the extraembryonic visceral endoderm layer during gastrulation, but it is found to be mutated in amnionless mouse.The encoded protein has sequence similarity to short gastrulation (Sog) and procollagen IIA proteins in Drosophila.Long isoforms are highly expressed in small intestine, colon and kidney (renal proximal tubule epithelial cells) . Shorter isoforms are detected at lower levels in testis, thymus and peripheral blood leukocytes.

Alternative Names: AMN; PRO1028; amnionless protein; visceral endoderm-specific type 1 transmembrane protein

Search name: AMN; PRO1028; amnionless protein; visceral endoderm-specific type 1 transmembrane protein

Tag: AMN