Human Protein kinase C-binding protein NELL2 (NELL2) ELISA Kit | KTE62220

Human Protein kinase C-binding protein NELL2 (NELL2) ELISA Kit | KTE62220 | Gentaur UK, US & Europe Distribution

Application: This Human Protein kinase C-binding protein NELL2 (NELL2) ELISA Kit employs a two-site sandwich ELISA to quantitate NELL2 in samples. An antibody specific for NELL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNELL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NELL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NELL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Protein kinase C-binding protein NELL2 microplate
• Human Protein kinase C-binding protein NELL2 standard
• Human Protein kinase C-binding protein NELL2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Protein kinase C-binding protein NELL2 (NELL2) ELISA Kit has high sensitivity and excellent specificity for detection of Human NELL2. No significant cross-reactivity or interference between Human NELL2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: NELL2 encodes a cytoplasmic protein that contains epidermal growth factor (EGF) -like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. An alternative splice variant has been described but its full length sequence has not been determined. NELL2 open reading frame encodes an 816-amino acid polypeptide. The amino acid sequence of NELL2 showed 80% identity to that of chicken 'nel, ' and Watanabe et al. (1996) stated that NELL2 may be the actual interspecies homolog of the 'nel' gene. NELL2 was strongly expressed as a 3.6-kb transcript in human adult and fetal brain tissues.

Alternative Names: NELL2; NRP2; NEL-like protein 2; NEL-related protein 2; neural epidermal growth factor-like 2; protein kinase C-binding protein NELL2

Search name: NELL2; NRP2; NEL-like protein 2; NEL-related protein 2; neural epidermal growth factor-like 2; protein kinase C-binding protein NELL2

Tag: NELL2