Human Protein NPAT (NPAT) ELISA Kit | KTE62363

Human Protein NPAT (NPAT) ELISA Kit | KTE62363 | Gentaur UK, US & Europe Distribution

Application: This Human Protein NPAT (NPAT) ELISA Kit employs a two-site sandwich ELISA to quantitate NPAT in samples. An antibody specific for NPAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNPAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPAT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Protein NPAT microplate
• Human Protein NPAT standard
• Human Protein NPAT detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Protein NPAT (NPAT) ELISA Kit has high sensitivity and excellent specificity for detection of Human NPAT. No significant cross-reactivity or interference between Human NPAT and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: NPAT lies only 0.5 kb from the 5-prime end of the ATM gene and is transcribed in the opposite direction as ATM.NPAT encodes a 1, 427-amino acid protein containing nuclear localization signals and target sites for phosphorylation by cyclin-dependent protein kinases associated with E2F. NPAT has a calculated molecular mass of 154, 300 Da. It is relatively serine and threonine rich. The mRNA of NPAT was detected in all human tissues examined and its genomic sequence was strongly conserved through eukaryotes, suggesting that the NPAT gene may be essential for cell maintenance, i.e., a housekeeping gene. The promoter region may be shared by ATM and NPAT and that each gene may influence the expression of the other.

Alternative Names: NPAT; E14; E14; nuclear protein; ataxia-telangiectasia locus

Search name: NPAT; E14; E14; nuclear protein; ataxia-telangiectasia locus

Tag: NPAT