Human Protein Wnt-10b (WNT10B) ELISA Kit | KTE60028

Human Protein Wnt-10b (WNT10B) ELISA Kit | KTE60028 | Gentaur UK, US & Europe Distribution

Application: This Human Protein Wnt-10b (WNT10B) ELISA Kit employs a two-site sandwich ELISA to quantitate WNT10B in samples. An antibody specific for WNT10B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWNT10B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT10B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT10B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Protein Wnt-10b microplate
• Human Protein Wnt-10b standard
• Human Protein Wnt-10b detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Protein Wnt-10b (WNT10B) ELISA Kit has high sensitivity and excellent specificity for detection of Human WNT10B. No significant cross-reactivity or interference between Human WNT10B and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: WNT10B is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. The human WNT10B sequence is 88% and 95% identical to the mouse gene at nucleotide and amino acid levels, respectively. WNT10B expression was not observed in normal and benign proliferations of human breast tissue but was found to be elevated in 3 of 50 primary breast carcinomas. Southern blot analysis of the carcinoma expressing the highest level of WNT10B showed no amplification or rearrangement of the gene.

Alternative Names: WNT10B; SHFM6; WNT-12; WNT-10B protein

Search name: WNT10B; SHFM6; WNT-12; WNT-10B protein

Tag: WNT10B