Human Proteinase 3 (PR3) ELISA Kit | KTE62338

Human Proteinase 3 (PR3) ELISA Kit | KTE62338 | Gentaur UK, US & Europe Distribution

Application: This Human Proteinase 3 (PR3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRTN3 in samples. An antibody specific for PRTN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRTN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRTN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRTN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Proteinase 3 microplate
• Human Proteinase 3 standard
• Human Proteinase 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Proteinase 3 (PR3) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRTN3. No significant cross-reactivity or interference between Human PRTN3 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PRTN3 is a serine protease enzyme expressed mainly in neutrophil granulocytes. Its exact role in the function of the neutrophil is unknown but in human neutrophils proteinase 3 contributes to the proteolytic generation of antimicrobial peptides. It is also the epitope of anti-neutrophil cytoplasmic antibodies (ANCAs) of the cANCA (cytoplasmic subtype) class, a type of antibody frequently found in the disease Wegener's granulomatosis. Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.

Alternative Names: PRTN3; ACPA; AGP7; C-ANCA; CANCA; MBN; MBT; NP4; P29; PR-3; PR3; Wegener granulomatosis autoantigen; azurophil granule protein 7; leukocyte proteinase 3; myeloblastin; serine proteinase; neutrophil

Search name: PRTN3; ACPA; AGP7; C-ANCA; CANCA; MBN; MBT; NP4; P29; PR-3; PR3; Wegener granulomatosis autoantigen; azurophil granule protein 7; leukocyte proteinase 3; myeloblastin; serine proteinase; neutrophil

Tag: PRTN3