Human Proteinase-antineutrophil cytoplasmic antibody (PR3-ANCA) ELISA Kit | AE63015HU

Human Proteinase-antineutrophil cytoplasmic antibody (PR3-ANCA) ELISA Kit | AE63015HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PR3-ANCA

Alternative Name: N/A

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.061 ng/mL

Intra-Assay: ≤4.7%

Inter-Assay: ≤7.9%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Qualitative

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PR3-ANCA in samples. An antibody specific for PR3-ANCA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPR3-ANCA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PR3-ANCA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PR3-ANCA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: PRTN3 is a serine protease enzyme expressed mainly in neutrophil granulocytes. Its exact role in the function of the neutrophil is unknown but in human neutrophils proteinase 3 contributes to the proteolytic generation of antimicrobial peptides. It is also the epitope of anti-neutrophil cytoplasmic antibodies (ANCAs) of the cANCA (cytoplasmic subtype) class, a type of antibody frequently found in the disease Wegener's granulomatosis.Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .