Human Proteoglycan 3 (PRG3) ELISA Kit | AE26036HU

Human Proteoglycan 3 (PRG3) ELISA Kit | AE26036HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PRG3

Alternative Name: MBP2; MBPH; MGC126662; MGC141971; prepro-major basic protein homolog

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤4.2%

Inter-Assay: ≤8.1%

Recovery: 1, 03

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PRG3 in samples. An antibody specific for PRG3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRG3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRG3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRG3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Through large-scale sequencing, Plager et al. (1999) identified PRG3 as one of several transcripts upregulated in umbilical cord precursor cells following stimulation with interleukin-5. Using this sequence as probe, Plager et al. (2001) cloned PRG3 from a phage genomic library of placental tissues. Plager et al. (1999) found that the cDNA sequence predicts a mature 117-amino acid protein with a prepro- region of 108 amino acids. PRG3 shares 49% amino acid sequence identity with PRG2. RT-PCR demonstrated that PRG3 is expressed exclusively in bone marrow. Protein purified from eosinophil granules showed a molecular mass of 13.4 kD.Plager et al. (1999) found that the biologic activity of PRG3 is similar to that of PRG2 in cell killing and in neutrophil and basophil stimulation assays, but usually with reduced potency.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .