Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) ELISA Kit | AE61304HU

Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) ELISA Kit | AE61304HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: DCD

Alternative Name: AIDD; DCD-1; DSEP; HCAP; MGC71930; PIF; diffusible survival/evasion peptide|preproteolysin|proteolysis inducing factor|survival promoting peptide

Application: ELISA

Range: 0.78-50 ng/mL

Sensitivity: 0.31 ng/mL

Intra-Assay: ≤5.1%

Inter-Assay: ≤10.3%

Recovery: 1, 05

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate DCD in samples. An antibody specific for DCD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDCD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Dermcidin is a secreted protein that is subsequently processed into mature peptides of distinct biological activities. The C-terminal peptide is constitutively expressed in sweat and has antibacterial and antifungal activities. The N-terminal peptide, also known as diffusible survival evasion peptide, promotes neural cell survival under conditions of severe oxidative stress. A glycosylated form of the N-terminal peptide may be associated with cachexia (muscle wasting) in cancer patients.High performance liquid chromatography, mass spectrometry, and microsequence analysis of sweat showed a processed 47-amino acid DCD peptide with a calculated mass of 4.7 kD, compared with the expected 9.3 kD of full-length DCD without the signal peptide.DCD expression in skin, melanocytic nevus, and cutaneous melanoma tissue, but not in any other organ systems tested.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .