Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) ELISA Kit | KTE62411

Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) ELISA Kit | KTE62411 | Gentaur UK, US & Europe Distribution

Application: This Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) ELISA Kit employs a two-site sandwich ELISA to quantitate DCD in samples. An antibody specific for DCD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDCD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Proteolysis Inducing Factor/Dermcidin microplate
• Human Proteolysis Inducing Factor/Dermcidin standard
• Human Proteolysis Inducing Factor/Dermcidin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) ELISA Kit has high sensitivity and excellent specificity for detection of Human DCD. No significant cross-reactivity or interference between Human DCD and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Dermcidin is a secreted protein that is subsequently processed into mature peptides of distinct biological activities. The C-terminal peptide is constitutively expressed in sweat and has antibacterial and antifungal activities. The N-terminal peptide, also known as diffusible survival evasion peptide, promotes neural cell survival under conditions of severe oxidative stress. A glycosylated form of the N-terminal peptide may be associated with cachexia (muscle wasting) in cancer patients.High performance liquid chromatography, mass spectrometry, and microsequence analysis of sweat showed a processed 47-amino acid DCD peptide with a calculated mass of 4.7 kD, compared with the expected 9.3 kD of full-length DCD without the signal peptide.DCD expression in skin, melanocytic nevus, and cutaneous melanoma tissue, but not in any other organ systems tested.

Alternative Names: DCD; AIDD; DCD-1; DSEP; HCAP; MGC71930; PIF; diffusible survival/evasion peptide; preproteolysin; proteolysis inducing factor; survival promoting peptide

Search name: DCD; AIDD; DCD-1; DSEP; HCAP; MGC71930; PIF; diffusible survival/evasion peptide; preproteolysin; proteolysis inducing factor; survival promoting peptide

Tag: DCD