Human Proto-oncogene serine/threonine-protein kinase pim-1 (PIM1) ELISA Kit | KTE62646

Human Proto-oncogene serine/threonine-protein kinase pim-1 (PIM1) ELISA Kit | KTE62646 | Gentaur UK, US & Europe Distribution

Application: This Human Proto-oncogene serine/threonine-protein kinase pim-1 (PIM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PIM1 in samples. An antibody specific for PIM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Proto-oncogene serine/threonine-protein kinase pim-1 microplate
• Human Proto-oncogene serine/threonine-protein kinase pim-1 standard
• Human Proto-oncogene serine/threonine-protein kinase pim-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Proto-oncogene serine/threonine-protein kinase pim-1 (PIM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PIM1. No significant cross-reactivity or interference between Human PIM1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Proto-oncogene serine/threonine-protein kinase Pim-1 is an enzyme encoded by the PIM1 gene. The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer (Dhanasekaran et al., 2001) .Viral leukemogenesis in mice is often initiated by proviral activation of the highly conserved cellular gene Pim1. Domen et al. (1987) compared the nucleotide sequence of human and mouse PIM1 cDNAs. Meeker et al. (1987) also characterized the human PIM1 gene. The deduced amino acid sequence showed significant homology to a number of the protein kinases but did not have a transmembrane region. Studies of expression of this gene using Northern blots of human cell lines showed it to be transcribed primarily in B-lymphoid and myeloid cell lines.

Alternative Names: PIM1; PIM; Oncogene PIM1; non-specific serine/threonine protein kinase; pim-1 kinase 44 kDa isoform; pim-1 oncogene (proviral integration site 1)

Search name: PIM1; PIM; Oncogene PIM1; non-specific serine/threonine protein kinase; pim-1 kinase 44 kDa isoform; pim-1 oncogene (proviral integration site 1)

Tag: PIM1