Human PSMa1 (Proteasome Subunit Alpha Type 1) ELISA Kit | ELK4359

Human PSMa1 (Proteasome Subunit Alpha Type 1) ELISA Kit | ELK4359 | Gentaur UK, US & Europe Distribution

Alternative Names: HC2; NU; PROS30; Proteasome (Prosome, Macropain) Subunit, Alpha Type, 1; Macropain subunit C2; Multicatalytic endopeptidase complex subunit C2; Proteasome nu chain

Species: Human

Assay Type: Sandwich

Sensitivity: 0.067 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteasome Subunit Alpha Type 1 (PSMa1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Proteasome Subunit Alpha Type 1 (PSMa1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteasome Subunit Alpha Type 1 (PSMa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proteasome Subunit Alpha Type 1 (PSMa1) in the samples is then determined by comparing the OD of the samples to the standard curve.