Human PTGIS (Prostaglandin I Synthase) ELISA Kit | ELK2052

Human PTGIS (Prostaglandin I Synthase) ELISA Kit | ELK2052 | Gentaur UK, US & Europe Distribution

Alternative Names: PGIS; CYP8A1; Prostacyclin Synthase; Prostacycline Synthetase; Prostagladin I2 Synthetase; PGI2 Synthetase; Cytochrome P450, Family 8, Subfamily A, Polypeptide 1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.61 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Cardiovascular biology;Hormone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin I Synthase (PTGIS) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prostaglandin I Synthase (PTGIS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin I Synthase (PTGIS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin I Synthase (PTGIS) in the samples is then determined by comparing the OD of the samples to the standard curve.