Human Pulmonary activation regulated chemokine (PARC) ELISA Kit | KTE62603

Human Pulmonary activation regulated chemokine (PARC) ELISA Kit | KTE62603 | Gentaur UK, US & Europe Distribution

Application: This Human Pulmonary activation regulated chemokine (PARC) ELISA Kit employs a two-site sandwich ELISA to quantitate CCL18 in samples. An antibody specific for CCL18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCCL18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL18 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL18 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Pulmonary activation regulated chemokine microplate
• Human Pulmonary activation regulated chemokine standard
• Human Pulmonary activation regulated chemokine detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Pulmonary activation regulated chemokine (PARC) ELISA Kit has high sensitivity and excellent specificity for detection of Human CCL18. No significant cross-reactivity or interference between Human CCL18 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines.Chemokine (C-C motif) ligand 18 (CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC (pulmonary and activation-regulated chemokine) . CCL18 is approximately 60% identical in amino acid sequence to CCL3. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes.The gene for human CCL18 contains three exons and is located on chromosome 17.

Alternative Names: CCL18; AMAC-1; AMAC1; CKb7; DC-CK1; DCCK1; MIP-4; PARC; SCYA18; CC chemokine ligand 18; alternative macrophage activation-associated CC chemokine 1; chemokine (C-C) ; dendritic; dendritic cell chemokine 1; macr

Search name: CCL18; AMAC-1; AMAC1; CKb7; DC-CK1; DCCK1; MIP-4; PARC; SCYA18; CC chemokine ligand 18; alternative macrophage activation-associated CC chemokine 1; chemokine (C-C) ; dendritic; dendritic cell chemokine 1; macr

Tag: CCL18