Human Putative annexin A2-like protein (ANXA2P2) ELISA Kit | KTE62441

Human Putative annexin A2-like protein (ANXA2P2) ELISA Kit | KTE62441 | Gentaur UK, US & Europe Distribution

Application: This Human Putative annexin A2-like protein (ANXA2P2) ELISA Kit employs a two-site sandwich ELISA to quantitate ANXA2P2 in samples. An antibody specific for ANXA2P2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyANXA2P2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANXA2P2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANXA2P2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Putative annexin A2-like protein microplate
• Human Putative annexin A2-like protein standard
• Human Putative annexin A2-like protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Putative annexin A2-like protein (ANXA2P2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ANXA2P2. No significant cross-reactivity or interference between Human ANXA2P2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Annexin 2 is involved in diverse cellular processes such as cell motility (especially that of the epithelial cells), linkage of membrane-associated protein complexes to the actin cytoskeleton, endocytosis, fibrinolysis, ion channel formation, and cell matrix interactions. It is a calcium-dependent phospholipid-binding protein whose function is to help organize exocytosis of intracellular proteins to the extracellular domain. Annexin II is a pleiotropic protein meaning that its function is dependent on place and time in the body. This protein is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption.

Alternative Names: ANXA2P2; ANX2L2; ANX2P2; LPC2B;

Search name: ANXA2P2; ANX2L2; ANX2P2; LPC2B;

Tag: ANXA2P2