Human PVRL4 (Poliovirus Receptor Related Protein 4) ELISA Kit | ELK2960

Human PVRL4 (Poliovirus Receptor Related Protein 4) ELISA Kit | ELK2960 | Gentaur UK, US & Europe Distribution

Alternative Names: PRR4; LNIR; Nectin-4; Ig superfamily receptor LNIR; Processed poliovirus receptor-related protein 4

Species: Human

Assay Type: Sandwich

Sensitivity: 51 pg/mL

Standard: 8000 pg/mL

Detection Range: 125-8000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Poliovirus Receptor Related Protein 4 (PVRL4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Poliovirus Receptor Related Protein 4 (PVRL4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Poliovirus Receptor Related Protein 4 (PVRL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Poliovirus Receptor Related Protein 4 (PVRL4) in the samples is then determined by comparing the OD of the samples to the standard curve.