Human Pygopus homolog 2 (PYGO2/PP7910) ELISA Kit | KTE61014

Human Pygopus homolog 2 (PYGO2/PP7910) ELISA Kit | KTE61014 | Gentaur UK, US & Europe Distribution

Application: This Human Pygopus homolog 2 (PYGO2/PP7910) ELISA Kit employs a two-site sandwich ELISA to quantitate PYGO2 in samples. An antibody specific for PYGO2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYGO2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYGO2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYGO2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Pygopus homolog 2 microplate
• Human Pygopus homolog 2 standard
• Human Pygopus homolog 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Pygopus homolog 2 (PYGO2/PP7910) ELISA Kit has high sensitivity and excellent specificity for detection of Human PYGO2. No significant cross-reactivity or interference between Human PYGO2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: WNT signaling controls many fundamental processes during animal development. WNT transduction is mediated by the association of beta-catenin with nuclear TCF DNA-binding factors. Lgs encodes the homolog of human BCL9, and the authors provided genetic and molecular evidence that these proteins exert their function by physically linking Pygo to beta-cateninThe PYGO1 and PYGO2 proteins, which contain 419 and 406 amino acids, respectively, possess a highly conserved PHD finger that interacts with homology domain-1 (HD1) of BCL9. The findings suggested that the recruitment of PYGO permits beta-catenin to transcriptionally activate WNT target genes and raised the possibility that a deregulation of these events may play a causal role in the development of B-cell malignancies.

Alternative Names: PYGO2; 1190004M21Rik; FLJ33226; pygopus 2; pygopus homolog 2

Search name: PYGO2; 1190004M21Rik; FLJ33226; pygopus 2; pygopus homolog 2

Tag: PYGO2