Human Pyroglutamylated RFamide peptide receptor (QRFPR) ELISA Kit | KTE61001

Human Pyroglutamylated RFamide peptide receptor (QRFPR) ELISA Kit | KTE61001 | Gentaur UK, US & Europe Distribution

Application: This Human Pyroglutamylated RFamide peptide receptor (QRFPR) ELISA Kit employs a two-site sandwich ELISA to quantitate QRFPR in samples. An antibody specific for QRFPR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQRFPR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QRFPR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QRFPR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Pyroglutamylated RFamide peptide receptor microplate
• Human Pyroglutamylated RFamide peptide receptor standard
• Human Pyroglutamylated RFamide peptide receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Pyroglutamylated RFamide peptide receptor (QRFPR) ELISA Kit has high sensitivity and excellent specificity for detection of Human QRFPR. No significant cross-reactivity or interference between Human QRFPR and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: GPR103 shares between 35% and 38% sequence identity in the transmembrane regions with various peptide receptors, including neuropeptide FF2, neuropeptide Y2, and galanin GalR1 receptors. Northern blot analysis of human brain regions revealed widespread expression, including transcripts of 4.0, 2.6 and 1.4 kb in the thalamus and hypothalamus, with a further 9.5-kb transcript in the hypothalamus and a 1.4-kb transcript in the pituitary.The deduced 431-amino acid protein contains the 7-transmembrane structure typical of G protein-coupled receptors. GPR103 shares 49% identity with NPFF1 (GPR147) and NPFF2 (GPR74) . Quantitative PCR analysis detected highest GPR103 expression in brain, predominantly in retina, trigeminal ganglion, hypothalamus, and vestibular nucleus

Alternative Names: QRFPR; AQ27; GPR103; MGC149217; SP9155; G protein-coupled receptor 103; OTTHUMP00000164030; QRFP receptor

Search name: QRFPR; AQ27; GPR103; MGC149217; SP9155; G protein-coupled receptor 103; OTTHUMP00000164030; QRFP receptor

Tag: QRFPR