Human RAB5A (RAB5A, Member RAS Oncogene Family) ELISA Kit | ELK4226

Human RAB5A (RAB5A, Member RAS Oncogene Family) ELISA Kit | ELK4226 | Gentaur UK, US & Europe Distribution

Alternative Names: RAB5

Species: Human

Assay Type: Sandwich

Sensitivity: 0.122 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAB5A, Member RAS Oncogene Family (RAB5A) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RAB5A, Member RAS Oncogene Family (RAB5A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAB5A, Member RAS Oncogene Family (RAB5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RAB5A, Member RAS Oncogene Family (RAB5A) in the samples is then determined by comparing the OD of the samples to the standard curve.