Human Ras-specific guanine nucleotide-releasing factor RalGPS1 (RALGPS1) ELISA Kit | KTE62337

Human Ras-specific guanine nucleotide-releasing factor RalGPS1 (RALGPS1) ELISA Kit | KTE62337 | Gentaur UK, US & Europe Distribution

Application: This Human Ras-specific guanine nucleotide-releasing factor RalGPS1 (RALGPS1) ELISA Kit employs a two-site sandwich ELISA to quantitate RALGPS1 in samples. An antibody specific for RALGPS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRALGPS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RALGPS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RALGPS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Ras-specific guanine nucleotide-releasing factor RalGPS1 microplate
• Human Ras-specific guanine nucleotide-releasing factor RalGPS1 standard
• Human Ras-specific guanine nucleotide-releasing factor RalGPS1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Ras-specific guanine nucleotide-releasing factor RalGPS1 (RALGPS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human RALGPS1. No significant cross-reactivity or interference between Human RALGPS1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: RalGPS1 is a Ral GEF that requires membrane targeting to function in vivo.RalGPS 1 is a highly specific Ral GEF. Guanine nucleotide exchange factors (GEFs) are responsible for coupling cell surface receptors to Ras protein activation. These molecules share the core catalytic domain of other Ras family GEFs but lack the catalytic non-conserved domain that is believed to contribute to Sos1 integrity. In vitro binding and in vivo nucleotide exchange assays indicate that these GEFs specifically catalyze the GTP loading of the Ral GTPase when overexpressed in 293T cells. A central proline-rich motif associated with the Src homology (SH) 2/SH3-containing adapter proteins Grb2 and Nck in vivo, whereas a pleckstrin homology (PH) domain was located at the GEF C terminus.

Alternative Names: RALGPS1; RP13-225O21.1; KIAA0351; RALGEF2; RALGPS1A; Ral guanine nucleotide exchange factor RalGPS1A

Search name: RALGPS1; RP13-225O21.1; KIAA0351; RALGEF2; RALGPS1A; Ral guanine nucleotide exchange factor RalGPS1A

Tag: RALGPS1