Human RBM20 (RNA Binding Motif Protein 20) ELISA Kit | ELK6644

Human RBM20 (RNA Binding Motif Protein 20) ELISA Kit | ELK6644 | Gentaur UK, US & Europe Distribution

Alternative Names: Rbm20; RBM20

Species: Human

Assay Type: Sandwich

Sensitivity: 0.114 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RNA Binding Motif Protein 20 (RBM20) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RNA Binding Motif Protein 20 (RBM20) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RNA Binding Motif Protein 20 (RBM20), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RNA Binding Motif Protein 20 (RBM20) in the samples is then determined by comparing the OD of the samples to the standard curve.