Human RCBTB2 (RCC1 And BTB Domain Containing Protein 2) ELISA Kit | ELK6887

Human RCBTB2 (RCC1 And BTB Domain Containing Protein 2) ELISA Kit | ELK6887 | Gentaur UK, US & Europe Distribution

Alternative Names: CHC1L; RLG; RCC1-like G exchanging factor; Regulator Of Chromosome Condensation And BTB (POZ) Domain Containing Protein 2; Chromosome Condensation 1-Like

Species: Human

Assay Type: Sandwich

Sensitivity: 0.124 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RCC1 And BTB Domain Containing Protein 2 (RCBTB2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RCC1 And BTB Domain Containing Protein 2 (RCBTB2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RCC1 And BTB Domain Containing Protein 2 (RCBTB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RCC1 And BTB Domain Containing Protein 2 (RCBTB2) in the samples is then determined by comparing the OD of the samples to the standard curve.