Human Receptor I for the Fc region of immunoglobulin A (FcARI) ELISA Kit | AE63090HU

Human Receptor I for the Fc region of immunoglobulin A (FcARI) ELISA Kit | AE63090HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: FcARI

Alternative Name: CD89; FcaR; Receptor I For The Fc Region Of Immunoglobulin A

Application: ELISA

Range: 0.78-50 ng/mL

Sensitivity: 0.37 ng/mL

Intra-Assay: ≤6.7%

Inter-Assay: ≤10.6%

Recovery: 0, 93

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate FcARI in samples. An antibody specific for FcARI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFcARI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FcARI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FcARI bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Only one Fc receptor belongs to the Fc alpha R subgroup, which is called Fc alpha RI (or CD89) . Fc alpha RI is found on the surface of neutrophils, eosinophils, monocytes, some macrophages (including Kupffer cells), and some dendritic cells. It is composed of two extracellular Ig-like domains, and is a member of both the immunoglobulin superfamily and the multi-chain immune recognition receptor (MIRR) family. It signals by associating with two FcR gamma，signaling chains.Another receptor can also bind IgA, although it has higher affinity for another antibody called IgM. This receptor is called the Fc-alpha/mu receptor (Fc alpha/mu R) and is a type I transmembrane protein. With one Ig-like domain in its extracellular portion, this Fc receptor is also a member of the immunoglobulin superfamily.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .