Human Receptor I for the Fc region of immunoglobulin A (FcARI) ELISA Kit | KTE62636

Human Receptor I for the Fc region of immunoglobulin A (FcARI) ELISA Kit | KTE62636 | Gentaur UK, US & Europe Distribution

Application: This Human Receptor I for the Fc region of immunoglobulin A (FcARI) ELISA Kit employs a two-site sandwich ELISA to quantitate FcARI in samples. An antibody specific for FcARI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFcARI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FcARI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FcARI bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Receptor I for the Fc region of immunoglobulin A microplate
• Human Receptor I for the Fc region of immunoglobulin A standard
• Human Receptor I for the Fc region of immunoglobulin A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Receptor I for the Fc region of immunoglobulin A (FcARI) ELISA Kit has high sensitivity and excellent specificity for detection of Human FcARI. No significant cross-reactivity or interference between Human FcARI and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Only one Fc receptor belongs to the Fc alpha R subgroup, which is called Fc alpha RI (or CD89) . Fc alpha RI is found on the surface of neutrophils, eosinophils, monocytes, some macrophages (including Kupffer cells), and some dendritic cells. It is composed of two extracellular Ig-like domains, and is a member of both the immunoglobulin superfamily and the multi-chain immune recognition receptor (MIRR) family. It signals by associating with two FcR gamma，signaling chains.Another receptor can also bind IgA, although it has higher affinity for another antibody called IgM. This receptor is called the Fc-alpha/mu receptor (Fc alpha/mu R) and is a type I transmembrane protein. With one Ig-like domain in its extracellular portion, this Fc receptor is also a member of the immunoglobulin superfamily.

Alternative Names: FcARI; CD89; FcaR; Receptor I For The Fc Region Of Immunoglobulin A

Search name: FcARI; CD89; FcaR; Receptor I For The Fc Region Of Immunoglobulin A

Tag: FcARI