Human Receptor I for the Fc region of immunoglobulin G (FcGRI) ELISA Kit | AE63093HU

Human Receptor I for the Fc region of immunoglobulin G (FcGRI) ELISA Kit | AE63093HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: FcGRI

Alternative Name: CD64; FCGR1; FCGR1A; FCGR1B; FCGR1C; FcgRIA; CD64A; FcgRIB; CD64B; FcgRIC; CD64C

Application: ELISA

Range: 78-5000 pg/mL

Sensitivity: 31 pg/mL

Intra-Assay: ≤5.6%

Inter-Assay: ≤9.8%

Recovery: 1, 08

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate FcGRI in samples. An antibody specific for FcGRI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFcGRI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FcGRI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FcGRI bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: CD64 is a type of integral membrane glycoprotein known as an Fc receptor that binds monomeric IgG-type antibodies with high affinity. It is more commonly known as Fc-gamma receptor 1 (Fc gamma RI) . After binding IgG, CD64 interacts with an accessory chain known as the common gamma chain (gamma chain), which possesses an ITAM motif that is necessary for triggering cellular activation.Structurally CD64 is composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail.CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFN gamma; and G-CSF can induce CD64 expression on these cells.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .