Human Receptor II for the Fc region of immunoglobulin G (FcGRII) ELISA Kit | KTE62638

Human Receptor II for the Fc region of immunoglobulin G (FcGRII) ELISA Kit | KTE62638 | Gentaur UK, US & Europe Distribution

Application: This Human Receptor II for the Fc region of immunoglobulin G (FcGRII) ELISA Kit employs a two-site sandwich ELISA to quantitate FcGRII in samples. An antibody specific for FcGRII has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFcGRII present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FcGRII is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FcGRII bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Receptor II for the Fc region of immunoglobulin G microplate
• Human Receptor II for the Fc region of immunoglobulin G standard
• Human Receptor II for the Fc region of immunoglobulin G detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Receptor II for the Fc region of immunoglobulin G (FcGRII) ELISA Kit has high sensitivity and excellent specificity for detection of Human FcGRII. No significant cross-reactivity or interference between Human FcGRII and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: CD32 is a surface receptor protein and part of a large population of B cell co-receptors. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.Monoclonal antibodies can distinguish between type A and type B.Receptors for the Fc portion of IgG such as FCGR2A play an essential role in the protection of the organism against foreign antigens by removing antigen-antibody complexes from the circulation. Receptors are present on monocytes, macrophages, neutrophils, natural killer (NK) cells, and T and B lymphocytes, and they participate in diverse functions such as phagocytosis of immune complexes and modulation of antibody production by B cells.

Alternative Names: FcGRII; CD32; FCGR2A; FCG2; FCGR2A1; FCGR2; FCGR2B; FCGR2C; Fc Fragment Of IgG, Low Affinitya, Receptor

Search name: FcGRII; CD32; FCGR2A; FCG2; FCGR2A1; FCGR2; FCGR2B; FCGR2C; Fc Fragment Of IgG, Low Affinitya, Receptor

Tag: FcGRII