Human Receptor III for the Fc region of immunoglobulin G (FcGRIII) ELISA Kit | AE63095HU

Human Receptor III for the Fc region of immunoglobulin G (FcGRIII) ELISA Kit | AE63095HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: FcGRIII

Alternative Name: N/A

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.135 ng/mL

Intra-Assay: ≤4.2%

Inter-Assay: ≤6.8%

Recovery: 0, 98

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate FcGRIII in samples. An antibody specific for FcGRIII has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFcGRIII present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FcGRIII is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FcGRIII bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Aitman et al. showed that copy number variation of the orthologous rat and human Fcgr3 genes is a determinant of susceptibility to immunologically mediated glomerulonephritis. Positional cloning identified loss of the rat-specific Fcgr3 paralog 'Fcgr3-related sequence' (Fcgr3rs) as a determinant of macrophage overactivity and glomerulonephritis in Wistar Kyoto rats. In humans, low copy number of FCGR3B, an ortholog of rat Fcgr3, was associated with glomerulonephritis in the autoimmune disorder systemic lupus erythematosus (SLE. Aitman et al. concluded that their finding that gene copy number polymorphism predisposes to immunologically mediated renal disease in 2 mammalian species provides direct evidence for the importance of genome plasticity in the evolution of genetically complex phenotypes, including susceptibility to common human disease.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .