Human Receptor-interacting serine/threonine-protein kinase 1 (RIPK1) ELISA Kit | AE22081HU

Human Receptor-interacting serine/threonine-protein kinase 1 (RIPK1) ELISA Kit | AE22081HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: RIPK1

Alternative Name: FLJ39204; RIP; RIP1; OTTHUMP00000015955|cell death protein RIP|receptor interacting protein|serine/threonine-protein kinase RIP

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.063 ng/mL

Intra-Assay: ≤3.9%

Inter-Assay: ≤8.0%

Recovery: 0, 9

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate RIPK1 in samples. An antibody specific for RIPK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRIPK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RIPK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RIPK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Receptor-interacting serine/threonine-protein kinase 1 is an enzyme that in humans is encoded by the RIPK1 gene. RIPK1 is known to have function in a variety of cellular pathways including the NF-κB pathway and programmed necrotic cell death (necroptosis)

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .