Human Recombinant ADA | 6393

ADA, also known as adenosine deaminase, catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. This protein plays an important role in purine metabolism and in adenosine homeostasis. ADA acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte epithelial cell adhesion.

6393 | Human Recombinant ADA DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant ADA

Alternates names: Adenosine deaminase, Adenosine aminohydrolase, ADA1.

Taglines: Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine

NCBI Gene ID #: 3586

NCBI Gene Symbol: IL10

Gene Source: Human

Accession #: P22301

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity: approximately >25 units/mg.

Results: >25 units/mg

Binding Capacity: N/A

Unit Definition: Enzymatic activity was confirmed by measuring the amount of enzyme that deaminates 1.0 µmol of adenosine to inosine per minute at pH 7.5 at 25°C.

Molecular Weight: 42.9 kDa (383 aa, 1-363 aa + NT His Tag)

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 20% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAQTPAFDKP KVELHVHLDG SIKPETILYY GRRRGIALPA NTAEGLLNVI GMDKPLTLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVEPIPWNQA EGDLTPDEVV ALVGQGLQEG ERDFGVKARS ILCCMRHQPN WSPKVVELCK KYQQQTVVAI DLAGDETIPG SSLLPGHVQA YQEAVKSGIH RTVHAGEVGS AEVVKEAVDI LKTERLGHGY HTLEDQALYN RLRQENMHFE ICPWSSYLTG AWKPDTEHAV IRLKNDQANY SLNTDDPLIF KSTLDTDYQM TKRDMGFTEE EFKRLNINAA KSSFLPEDEK RELLDLLYKA YGMPPSASAG QNL