Human Recombinant AK2 | 6386

Adenylate kinase (AK; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phosphotransferase) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (AK1, AK2 and AK3) are characterized in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis.

6386 | Human Recombinant AK2 DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant AK2

Alternates names: Adenylate kinase 2, ADK2

Taglines: Involved energy metabolism of prokaryotic and eukaryotic cells

NCBI Gene ID #: 3586

NCBI Gene Symbol: IL10

Gene Source: Human

Accession #: P22301

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 g of protein (determined by LAL method)

Activity (Specifications/test method): N/A

Biological activity: Specific activity: > 1.5 units/ml.

Results: >1.5 units/ml

Binding Capacity: NA

Unit Definition: One unit will convert 2.0 µmoles of ADP to ATP + AMP per minute at pH 7.5 at 25°C.

Molecular Weight: 28.6 kDa (259 aa, 1-239 aa + NT His Tag)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 7.5) containing 5 mM DTT and 20% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAPSVPAAEP EYPKGIRAVL LGPPGAGKGT QAPRLAENFC VCHLATGDML RAMVASGSEL GKKLKATMDA GKLVSDEMVV ELIEKNLETP LCKNGFLLDG FPRTVRQAEM LDDLMEKRKE KLDSVIEFSI PDSLLIRRIT GRLIHPKSGR SYHEEFNPPK EPMKDDITGE PLIRRSDDNE KALKIRLQAY HTQTTPLIEY YRKRGIHSAI DASQTPDVVF ASILAAFSKA TCKDLVMFI