Human Recombinant ALDH2 | 6332

ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. ALDH2 has been shown to interact with GroEL.

6332 | Human Recombinant ALDH2 DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant ALDH2

Alternates names: Aldehyde dehydrogenase - mitochondrial, ALDH-E2, ALDHI, ALDM.

Taglines: A polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids

NCBI Gene ID #: 25325

NCBI Gene Symbol: IL10

Gene Source: Human

Accession #: P29456

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 g of protein (determined by LAL method).

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 0.14 units/ml

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit will oxidize 1.0 µmole of acetaldehyde to acetic acid per minute at pH 8.0 at 25°C in the presence of beta-NAD, potassium and thiols.

Molecular Weight: 54.5 kDa

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 1 mM EDTA and 10% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S