Human Recombinant Alpha-Enolase | 6363

Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an auto antigen associated with Hashimoto's encephalopathy and severe asthma.

6363 | Human Recombinant Alpha-Enolase DataSheet

Biomolecule/Target: ENO1

Synonyms: Human Recombinant Alpha-Enolase

Alternates names: Peptidyl-prolyl cis-trans isomerase-like 4, HDCME13P, Rotamase PPIL4, Cyclophilin-like protein PPIL4.

Taglines: A metalloenzyme responsible for conversion of 2-PG to PEP

NCBI Gene ID #: 85313

NCBI Gene Symbol: PPIL4

Gene Source: Human

Accession #: Q8WUA2

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 85%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 190 nmoles/min/mg

Results:

Binding Capacity: N/A

Unit Definition: One unit is the amount of enzyme that will generate 1.0 nmole of H2O2 per minute at pH 7.2 and 25 C.

Molecular Weight: 59.4 kDa

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: In 50 mM Tris, 150 mM NaCl, 20 mM MgCl2 and 20% Glycerol

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAVLLETTLG DVVIDLYTEE RPRACLNFLK LCKIKYYNYC LIHNVQRDFI IQTGDPTGTG RGGESIFGQL YGDQASFFEA EKVPRIKHKK KGTVSMVNNG SDQHGSQFLI TTGENLDYLD GVHTVFGEVT EGMDIIKKIN ETFVDKDFVP YQDIRINHTV ILDDPFDDPP DLLIPDRSPE PTREQLDSGR IGADEEIDDF KGRSAEEVEE IKAEKEAKTQ AILLEMVGDL PDADIKPPEN VLFVCKLNPV TTDEDLEIIF SRFGPIRSCE VIRDWKTGES LCYAFIEFEK EEDCEKAFFK MDNVLIDDRR IHVDFSQSVA KVKWKGKGGK YTKSDFKEYE KEQDKPPNLV LKDKVKPKQD TKYDLILDEQ AEDSKSSHSH TSKKHKKKTH HCSEEKEDED YMPIKNTNQD IYREMGFGHY EEEESCWEKQ KSEKRDRTQN RSRSRSRERD GHYSNSHKSK YQTDLYERER SKKRDRSRSP KKSKDKEKSK YR