Human Recombinant Beta-Enolase | 6364

Beta-enolase, also known as ENO3, is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. ENO3 play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. Mutations in its gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Recombinant human ENO3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

6364 | Human Recombinant Beta-Enolase DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant Beta-Enolase

Alternates names: ENO3, MSE

Taglines: A metalloenzyme responsible for conversion of 2-PG to PEP

NCBI Gene ID #: 3589

NCBI Gene Symbol: IL11

Gene Source: Human

Accession #: P20809

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity: >1.5 units/ml.

Results: >1.5 units/ml

Binding Capacity: N/A

Unit Definition: One unit will convert 1.0 µmole of 2-phosphoglycerate to phosphoenol pyruvate per minute at pH 7.5 at 25°C.

Molecular Weight: 49 kDa (454 aa, 1-434 aa + NT His Tag)

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml solution in 20 mM Tris-HCl (pH 8.0) containing 1 mM DTT, 20% glycerol and 0.1 mM NaCl.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAMQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR DGDKGRYLGK GVLKAVENIN STLGPALLQK KLSVADQEKV DKFMIELDGT ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN PDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGASSFKE AMRIGAEVYH HLKGVIKAKY GKDATNVGDE GGFAPNILEN NEALELLKTA IQAAGYPDKV VIGMDVAASE FYRNGKYDLD FKSPDDPARH ITGEKLGelY KSFIKNYPVV SIEDPFDQDD WATWTSFLSG VNIQIVGDDL TVTNPKRIAQ AVEKKACNCL LLKVNQIGSV TESIQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEALGDK AIFAGRKFRN PKAK