Human Recombinant PAI-1 | 6377

Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator (tPA) and Urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots). It is a serine protease inhibitor (serpin) protein (SERPINE1). The other PAI, plasminogen activator inhibitor-2 (PAI-2) is secreted by the placenta and only present in significant amounts during pregnancy. In addition, protease nexin acts as an inhibitor of tPA and urokinase. SERPINE1, however, is the main inhibitor of the plasminogen activators.

6377 | Human Recombinant PAI-1 DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant PAI-1

Alternates names: Plasminogen activator inhibitor-1, SERPINE1, PAI, PAI1, PLANH1.

Taglines: Activator of plasminogen and fibrinolysis

NCBI Gene ID #: 112744

NCBI Gene Symbol: IL24

Gene Source: Human

Accession #: Q96PD4

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity, the IC50 value is < 56 nM. Measured by its ability to inhibit uPA cleavage of the substrate Z-GGR-AMC.

Results: <56 nM

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 45 kDa (400 aa, 24-402 aa + NT His-Tag)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 150 mM Na2HPO4 (pH 6.6) containing 0.5 mM NaCl, 2 mM Glutathione and 0.01% Tween-80.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MVHHPPSYVA HLASDFGVRV FQQVAQASKD RNVVFSPYGV ASVLAMLQLT TGGETQQQIQ AAMGFKIDDK GMAPALRHLY KELMGPWNKD EISTTDAIFV QRDLKLVQGF MPHFFRLFRS TVKQVDFSEV ERARFIINDW VKTHTKGMIS NLLGKGAVDQ LTRLVLVNAL YFNGQWKTPF PDSSTHRRLF HKSDGSTVSV PMMAQTNKFN YTEFTTPDGH YYDILELPYH GDTLSMFIAA PYEKEVPLSA LTNILSAQLI SHWKGNMTRL PRLLVLPKFS LETEVDLRKP LENLGMTDMF RQFQADFTSL SDQEPLHVAQ ALQKVKIEVN ESGTVASSST AVIVSARMAP EEIIMDRPFL FVVRHNPTGT VLFMGQVMEP