Human Recombinant PP2C alpha | 6303

Protein phosphatase 2C (PP2C alpha) is a Mn2+- or Mg2+- dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2C alpha (amino acids 1-382) was cloned into an E. coli expression vector. PP2C alpha was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques.

6303 | Human Recombinant PP2C alpha DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant PP2C alpha

Alternates names: Protein phosphatase 1A (formerly 2C), human recombinant PP2C alpha, hrPP2C alpha, human PP2C alpha, recombinant PP2C alpha, magnesium-dependent, alpha isoform, PPM1A, PP2CA, PP2C-ALPHA.

Taglines: Negative regulator of cell stress response pathways

NCBI Gene ID #: 140806

NCBI Gene Symbol: IL-17E

Gene Source: Human

Accession #: Q8VHH8

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 g of protein (determined by LAL method)

Activity (Specifications/test method): N/A

Biological activity: >8,000 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: Enzymatic activity is defined as the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37°C, pH 7.5 using 10 mM of substrate.

Molecular Weight: 46.6 kDa (418aa)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 2 mM DTT, 1 mM MnCl2, 20% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWIL FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC MGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGelASK RNVIEAVYNR LNPYKNDDTD STSTDDMW