Human Recombinant TNF-beta/LTA | 6376

TNF-beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. It is highly inducible, secreted, and exists as homotrimeric molecule. It forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. It mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. It is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.

6376 | Human Recombinant TNF-beta/LTA DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant TNF-beta/LTA

Alternates names: Lymphotoxin alpha, LT, TNFB, TNFSF1.

Taglines: Mediates a large variety of inflammatory, immunostimulatory, and antiviral responses

NCBI Gene ID #: 59067

NCBI Gene Symbol: IL21

Gene Source: Human

Accession #: Q9HBE4

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is <0.3 ng/ml.

Results: <0.3 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 20.9 kDa (192 aa, 35-205 aa + His-Tag)

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL