Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein (REG1A) ELISA Kit | AE22529HU

Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein (REG1A) ELISA Kit | AE22529HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: REG1A

Alternative Name: ICRF; MGC12447; P19; PSP; PSPS; PSPS1; PTP; REG; islet cells regeneration factor|islet of langerhans regenerating protein|lithostathine 1 alpha|pancreatic stone protein; secretory|pancreatic thread

Application: ELISA

Range: 62.5-4000 pg/mL

Sensitivity: 24.4 pg/mL

Intra-Assay: ≤5.9%

Inter-Assay: ≤9.1%

Recovery: 0, 92

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate REG1A in samples. An antibody specific for REG1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyREG1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REG1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REG1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Pancreatic stone protein is the major component of the protein matrix of calculi in patients suffering from chronic calcifying pancreatitis. Secretory pancreatic stone protein is a glycoprotein in the pancreatic secretion. This protein, called PSPS, occurs in multiple molecular forms due to posttranslational processing. The abundance of PSPS in pancreatic juice (10 to 14% of total protein) suggests that it plays an important role in exocrine pancreatic function. In vitro experiments show that PSPS inhibits CaCO (3) crystal growth. Since in all the pancreatic secretions are supersaturated in calcium carbonate, the physiologic role of PSPS may be related to its inhibitory properties. This gene encodes a protein that is secreted by the exocrine pancreas.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .