Human Ribonucleoside-diphosphate reductase large subunit (RRM1) ELISA Kit | AE21160HU

Human Ribonucleoside-diphosphate reductase large subunit (RRM1) ELISA Kit | AE21160HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: RRM1

Alternative Name: R1; RIR1; RR1; ribonucleoside-diphosphate reductase M1 chain|ribonucleotide reductase M1 polypeptide|ribonucleotide reductase; R1 subunit|ribonucleotide reductase; large subunit

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.115 ng/mL

Intra-Assay: ≤4.8%

Inter-Assay: ≤9.1%

Recovery: 0, 9

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate RRM1 in samples. An antibody specific for RRM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRRM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RRM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RRM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: RRM1 encodes one of two non-identical subunits which constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. This gene is oriented in a head-to-tail configuration with the stromal interaction molecule 1 gene (STIM1), with the 3' end of STIM1 situated 1.6 kb from the 5' end of this gene.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .