Human RNASE1 (Ribonuclease A) ELISA Kit | ELK1930

Human RNASE1 (Ribonuclease A) ELISA Kit | ELK1930 | Gentaur UK, US & Europe Distribution

Alternative Names: RNASE; Rnase-A; RNase UpI-1; RIB-1; Ribonuclease, RNase A Family, 1 (Pancreatic)

Species: Human

Assay Type: Sandwich

Sensitivity: 31 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A (RNASE1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ribonuclease A (RNASE1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A (RNASE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribonuclease A (RNASE1) in the samples is then determined by comparing the OD of the samples to the standard curve.