Human Rock2 (Rho Associated Coiled Coil Containing Protein Kinase 2) ELISA Kit | ELK7603

Human Rock2 (Rho Associated Coiled Coil Containing Protein Kinase 2) ELISA Kit | ELK7603 | Gentaur UK, US & Europe Distribution

Alternative Names: KIAA0619 ; p164 ROCK 2 ; p164 ROCK-2 ; Rho kinase 2 ; Rho-associated ; Rho-associated protein kinase 2 ; ROCK 2 ; Rock II ; Rock2 ; ROCK2; Rock2m ; ROK alpha ; ROKalpha

Species: Human

Assay Type: Sandwich

Sensitivity: 0.119 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Enzyme & Kinase;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) in the samples is then determined by comparing the OD of the samples to the standard curve.