855
Human RXFP3 (Relaxin/Insulin Like Family Peptide Receptor 3) ELISA Kit | ELK4343
- SKU:
- 855-ELK4343-GEN
Description
Human RXFP3 (Relaxin/Insulin Like Family Peptide Receptor 3) ELISA Kit | ELK4343 | Gentaur UK, US & Europe Distribution
Alternative Names: GPCR135; RLN3R1; SALPR; RXFPR3; Relaxin 3 Receptor 1; G-protein coupled receptor GPCR135; Somatostatin- and angiotensin-like peptide receptor
Species: Human
Assay Type: Sandwich
Sensitivity: 0.064 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Endocrinology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Relaxin/Insulin Like Family Peptide Receptor 3 (RXFP3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Relaxin/Insulin Like Family Peptide Receptor 3 (RXFP3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Relaxin/Insulin Like Family Peptide Receptor 3 (RXFP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Relaxin/Insulin Like Family Peptide Receptor 3 (RXFP3) in the samples is then determined by comparing the OD of the samples to the standard curve.