Human S100A16 (S100 Calcium Binding Protein A16) ELISA Kit | ELK5130

Human S100A16 (S100 Calcium Binding Protein A16) ELISA Kit | ELK5130 | Gentaur UK, US & Europe Distribution

Alternative Names: S100-A16; AAG13; DT1P1A7; S100F; S100-F; Aging-associated gene 13 protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.069 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A16 (S100A16) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A16 (S100A16) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A16 (S100A16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A16 (S100A16) in the samples is then determined by comparing the OD of the samples to the standard curve.