Human S100A2 (S100 Calcium Binding Protein A2) ELISA Kit | ELK3988

Human S100A2 (S100 Calcium Binding Protein A2) ELISA Kit | ELK3988 | Gentaur UK, US & Europe Distribution

Alternative Names: S100-A2; CAN19; S100L

Species: Human

Assay Type: Sandwich

Sensitivity: 0.113 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity;Infection immunity;Reproductive science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A2 (S100A2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A2 (S100A2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A2 (S100A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A2 (S100A2) in the samples is then determined by comparing the OD of the samples to the standard curve.