Human S100A7 (S100 Calcium Binding Protein A7) ELISA Kit | ELK2998

Human S100A7 (S100 Calcium Binding Protein A7) ELISA Kit | ELK2998 | Gentaur UK, US & Europe Distribution

Alternative Names: S100-A7; PSOR1; S100A7c; Psoriasin 1

Species: Human

Assay Type: Sandwich

Sensitivity: 50 pg/mL

Standard: 8000 pg/mL

Detection Range: 125-8000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A7 (S100A7) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A7 (S100A7) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A7 (S100A7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A7 (S100A7) in the samples is then determined by comparing the OD of the samples to the standard curve.