Human SAA4 (Serum Amyloid A4, Constitutive) ELISA Kit | ELK4953

Human SAA4 (Serum Amyloid A4, Constitutive) ELISA Kit | ELK4953 | Gentaur UK, US & Europe Distribution

Alternative Names: C-SAA; CSAA; SA-A4; Constitutively expressed serum amyloid A protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.063 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serum Amyloid A4, Constitutive (SAA4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Serum Amyloid A4, Constitutive (SAA4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serum Amyloid A4, Constitutive (SAA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serum Amyloid A4, Constitutive (SAA4) in the samples is then determined by comparing the OD of the samples to the standard curve.