Human SCT (Secretin) ELISA Kit | ELK1753

Human SCT (Secretin) ELISA Kit | ELK1753 | Gentaur UK, US & Europe Distribution

Alternative Names: Secretin

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 7.14 pg/mL

Standard: 1500 pg/mL

Detection Range: 23.44-1500 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Endocrinology;Hepatology;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Secretin (SCT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Secretin (SCT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secretin (SCT) in the samples is then determined by comparing the OD of the samples to the standard curve.