Human SCUBE1 (Signal peptide, CUB and EGF-like domain-containing protein 1) ELISA Kit | ELK9465

Human SCUBE1 (Signal peptide, CUB and EGF-like domain-containing protein 1) ELISA Kit | ELK9465 | Gentaur UK, US & Europe Distribution

Alternative Names: SCUB1_HUMAN antibody; SCUBE 1 antibody; SCUBE1 antibody; Signal peptide, CUB and EGF-like domain-containing protein 1 antibody; Signal peptide, CUB domain, EGF like 1 antibody

Species: Human

Assay Type: Sandwich

Sensitivity: 0.094 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cardiovascular

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal peptide, CUB and EGF-like domain-containing protein 1 (SCUBE1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Signal peptide, CUB and EGF-like domain-containing protein 1 (SCUBE1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal peptide, CUB and EGF-like domain-containing protein 1 (SCUBE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Signal peptide, CUB and EGF-like domain-containing protein 1 (SCUBE1) in the samples is then determined by comparing the OD of the samples to the standard curve.