Human SDF1 (Stromal Cell Derived Factor 1) ELISA Kit | ELK1183

Human SDF1 (Stromal Cell Derived Factor 1) ELISA Kit | ELK1183 | Gentaur UK, US & Europe Distribution

Alternative Names: CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12

Species: Human

Assay Type: Sandwich

Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stromal Cell Derived Factor 1 (SDF1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Stromal Cell Derived Factor 1 (SDF1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stromal Cell Derived Factor 1 (SDF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stromal Cell Derived Factor 1 (SDF1) in the samples is then determined by comparing the OD of the samples to the standard curve.