Human SELL (Selectin, Leukocyte) ELISA Kit | ELK5204

Human SELL (Selectin, Leukocyte) ELISA Kit | ELK5204 | Gentaur UK, US & Europe Distribution

Alternative Names: CD62L; L-Selectin; SEL-L; LAM1; LECAM1; LNHR; LSEL; LYAM1; Leu-8; Lyam-1; PLNHR; TQ1; HLHRc; MEL14; Lymphocyte Adhesion Molecule 1; Lymph Node Homing Receptor

Species: Human

Assay Type: Sandwich

Sensitivity: 0.49 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selectin, Leukocyte (SELL) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Selectin, Leukocyte (SELL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Selectin, Leukocyte (SELL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Selectin, Leukocyte (SELL) in the samples is then determined by comparing the OD of the samples to the standard curve.