Human Semaphorin-4B (SEMA4B) ELISA Kit | AE20174HU

Human Semaphorin-4B (SEMA4B) ELISA Kit | AE20174HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: SEMA4B

Alternative Name: KIAA1745; MGC131831; SEMAC; SemC; sema domain; immunoglobulin domain (Ig) ; transmembrane domain (TM) and short cytoplasmic domain; 4B|semaphorin 4B

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.071 ng/mL

Intra-Assay: ≤6.1%

Inter-Assay: ≤8.9%

Recovery: 1

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SEMA4B in samples. An antibody specific for SEMA4B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA4B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA4B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA4B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Semaphorin-4B is a protein encoded by the SEMA4B gene. Members of the collapsin/semaphorin gene family have been proposed to act as growth cone guidance signals in vertebrates and invertebrates. To identify candidate molecules involved in axonal pathfinding during mouse embryogenesis, isolated cDNAs encoding five new members of the semaphorin family (Sem A-Sem E) . The murine semaphorin genes are differentially expressed in mesoderm and neuroectoderm before and during the time when axons select their pathways in the embryo. In explant cultures, recombinant Sem D/collapsin converts a matrix permissive for axonal growth into one that is inhibitory for neurites of peripheral ganglia. Semaphorins are a diverse family of molecules that may provide local signals to specify territories nonaccessible for growing axons.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .